Digoxigenin-Labeled <i>In Situ</i> Hybridization for the Detection of <i>Streptococcus suis</i> DNA in Polyserositis and a Comparison with Biotinylated <i>In Situ</i> Hybridization
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منابع مشابه
Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization
The objective of this study was to develop digoxigenin-labeled in situ hybridization (ISH) for the detection of Streptococcus suis in naturally infected pigs with polyserositis and to compare it with biotinylated ISH. Digoxigenin-labeled hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis and microcolonies in the blood vessels. Mock hybridizat...
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CONTENTS Abstract We describe the use of PCR to generate sense and antisense template DNA for nonradioactive in vitro transcription reactions. The resulting digoxigenin-labeled RNA probes can be used in a small-scale Northern hybridization procedure that is performed in microcentrifuge tubes. This kind of Northern approach is especially useful when the expression of several genes is to be initi...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2014
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.13-0204